Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby.
Identifieur interne : 001D96 ( Main/Exploration ); précédent : 001D95; suivant : 001D97Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby.
Auteurs : Xu Lu [République populaire de Chine] ; Wei Zhou ; Feng GaoSource :
- Molecular biology reports [ 1573-4978 ] ; 2009.
English descriptors
- KwdEn :
- Acyltransferases (chemistry), Acyltransferases (genetics), Amino Acid Sequence, Chromosomes, Plant (enzymology), Citrus sinensis (enzymology), Citrus sinensis (genetics), Cloning, Molecular, Computational Biology, DNA, Plant (genetics), Genes, Plant, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phylogeny, Protein Structure, Secondary, Sequence Alignment, Sequence Homology, Amino Acid.
- MESH :
- chemical , chemistry : Acyltransferases.
- chemical , genetics : Acyltransferases, DNA, Plant.
- enzymology : Chromosomes, Plant, Citrus sinensis.
- genetics : Citrus sinensis.
- Amino Acid Sequence, Cloning, Molecular, Computational Biology, Genes, Plant, In Situ Hybridization, Fluorescence, Molecular Sequence Data, Phylogeny, Protein Structure, Secondary, Sequence Alignment, Sequence Homology, Amino Acid.
Abstract
Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that at least three copies of CsCHS-bo were present in the genome.
DOI: 10.1007/s11033-008-9408-z
PubMed: 19005777
Affiliations:
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Le document en format XML
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Ruby.</title><author><name sortKey="Lu, Xu" sort="Lu, Xu" uniqKey="Lu X" first="Xu" last="Lu">Xu Lu</name><affiliation wicri:level="3"><nlm:affiliation>School of Life Sciences, South China Normal University, Guangzhou, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Life Sciences, South China Normal University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhou, Wei" sort="Zhou, Wei" uniqKey="Zhou W" first="Wei" last="Zhou">Wei Zhou</name></author><author><name sortKey="Gao, Feng" sort="Gao, Feng" uniqKey="Gao F" first="Feng" last="Gao">Feng Gao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19005777</idno><idno type="pmid">19005777</idno><idno type="doi">10.1007/s11033-008-9408-z</idno><idno type="wicri:Area/PubMed/Corpus">000930</idno><idno type="wicri:Area/PubMed/Curation">000930</idno><idno type="wicri:Area/PubMed/Checkpoint">000930</idno><idno type="wicri:Area/Ncbi/Merge">000915</idno><idno type="wicri:Area/Ncbi/Curation">000915</idno><idno type="wicri:Area/Ncbi/Checkpoint">000915</idno><idno type="wicri:Area/Main/Merge">001E45</idno><idno type="wicri:Area/Main/Curation">001D96</idno><idno type="wicri:Area/Main/Exploration">001D96</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning, characterization and localization of CHS gene from blood orange, Citrus sinensis (L.) Osbeck cv. Ruby.</title><author><name sortKey="Lu, Xu" sort="Lu, Xu" uniqKey="Lu X" first="Xu" last="Lu">Xu Lu</name><affiliation wicri:level="3"><nlm:affiliation>School of Life Sciences, South China Normal University, Guangzhou, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Life Sciences, South China Normal University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhou, Wei" sort="Zhou, Wei" uniqKey="Zhou W" first="Wei" last="Zhou">Wei Zhou</name></author><author><name sortKey="Gao, Feng" sort="Gao, Feng" uniqKey="Gao F" first="Feng" last="Gao">Feng Gao</name></author></analytic><series><title level="j">Molecular biology reports</title><idno type="eISSN">1573-4978</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acyltransferases (chemistry)</term><term>Acyltransferases (genetics)</term><term>Amino Acid Sequence</term><term>Chromosomes, Plant (enzymology)</term><term>Citrus sinensis (enzymology)</term><term>Citrus sinensis (genetics)</term><term>Cloning, Molecular</term><term>Computational Biology</term><term>DNA, Plant (genetics)</term><term>Genes, Plant</term><term>In Situ Hybridization, Fluorescence</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Protein Structure, Secondary</term><term>Sequence Alignment</term><term>Sequence Homology, Amino Acid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Acyltransferases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Acyltransferases</term><term>DNA, Plant</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Chromosomes, Plant</term><term>Citrus sinensis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Cloning, Molecular</term><term>Computational Biology</term><term>Genes, Plant</term><term>In Situ Hybridization, Fluorescence</term><term>Molecular Sequence Data</term><term>Phylogeny</term><term>Protein Structure, Secondary</term><term>Sequence Alignment</term><term>Sequence Homology, Amino Acid</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chalcone synthase (CHS) is involved in the biosynthesis of anthocyanin. In this study, a full-length DNA of CHS gene (named as CsCHS-bo) was cloned from the blood orange, Citrus sinensis (L.) Osbeck cv. Ruby. The gene was 1,512 bp in size containing an open reading frame (1,176 bp) encoding 391 amino acids. Comparative and bioinformatic analyses revealed that the deduced protein of CsCHS-bo was highly homologous to CHS from other plant species. The protein of CsCHS-bo had four CHS-specific conserved motifs and a CHS-family signature sequence GFGPG. Phylogenetic analysis indicated that the protein of CsCHS-bo was in a subgroup with CHS of Ruta Palmatum. The CsCHS-bo was localized to the chromosomes 2p, 4p and 6p by an improved fluorescence in situ hybridization technique, indicating that at least three copies of CsCHS-bo were present in the genome.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Gao, Feng" sort="Gao, Feng" uniqKey="Gao F" first="Feng" last="Gao">Feng Gao</name><name sortKey="Zhou, Wei" sort="Zhou, Wei" uniqKey="Zhou W" first="Wei" last="Zhou">Wei Zhou</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Lu, Xu" sort="Lu, Xu" uniqKey="Lu X" first="Xu" last="Lu">Xu Lu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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